Peter Parker/Spider-Man (voice)

Josh Keaton

Acting

Gwen Stacy (voice)

Lacey Chabert

Acting

Harry Osborn (voice)

James Arnold Taylor

Acting

Norman Osborn (voice)

Alan Rachins

Acting

Aunt May Parker (voice)

Deborah Strang

Acting

Eddie Brock (voice)

Ben Diskin

Acting

Curt Connors/Lizard (voice)

Dee Bradley Baker

Acting

Adrian Toomes/Vulture (voice)

Robert Englund

Acting

Max Dillon/Electro (voice)

Crispin Freeman

Acting

Montana/Shocker (voice)

Jeff Bennett

Acting

J. Jonah Jameson (voice)

Daran Norris

Acting

Flash Thompson (voice)

Joshua LeBar

Acting

Otto Octavius/Doctor Octopus (voice)

Peter MacNicol

Acting

Liz Allen (voice)

Alanna Ubach

Acting

Sally Avril (voice), Betty Brant (voice)

Grey DeLisle

Acting

Alex O'Hirn/Rhino (voice)

Clancy Brown

Acting