Mary Stuart

Katharine Hepburn

Acting

Elizabeth Tudor

Florence Eldridge

Acting

Bothwell

Fredric March

Acting

Darnley

Douglas Walton

Acting

Rizzio

John Carradine

Acting

Morton

Robert Barrat

Acting

Leicester

Gavin Muir

Acting

Moray

Ian Keith

Acting

John Knox

Moroni Olsen

Acting

Ruthven

William Stack

Acting

Randolph

Ralph Forbes

Acting

Throckmorton

Alan Mowbray

Acting

Mary Beaton

Frieda Inescort

Acting

Huntly

Donald Crisp

Acting

Lindsay

David Torrence

Acting

Mary Livingstone

Molly Lamont

Acting

Mary Fleming

Anita Colby

Acting

Mary Seton

Jean Fenwick

Acting

Burghley

Lionel Pape

Acting

Donal

Alec Craig

Acting

Nurse

Mary Gordon

Acting

Messenger

Monte Blue

Acting

Sir Francis Knollys

Robert Warwick

Acting

Man (uncredited)

Halliwell Hobbes

Acting

Judge

Nigel De Brulier

Acting