Scientist

Lugosi Béla

Acting

Glen / Glenda (as Daniel Davis)

Edward D. Wood Jr.

Writing

Inspector Warren

Lyle Talbot

Acting

Dr. Alton / Narrator

Timothy Farrell

Acting

Barbara

Dolores Fuller

Acting

Alan / Anne

'Tommy' Haynes

Acting

Johnny (as Charles Crafts)

Charlie Crafts

Acting

Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Conrad Brooks

Acting

Man with Hat and Receding Hairline (uncredited)

Henry Bederski

Acting

Woman in Nightmare (uncredited)

Carol Daugherty

Acting

The Devil / Glen's Father (uncredited)

'Captain' DeZita

Acting

(uncredited)

Helen Miles

Acting

Miss Stevens (uncredited)

Shirley Speril

Acting

Minor Role (uncredited)

Amzie Strickland

Acting

Man in Nightmare (uncredited)

Harry Thomas

Costume & Make-Up

Judge (uncredited)

William C. Thompson

Camera

Patrick / Patricia (uncredited)

'Mr. Walter' Hadjwiecyz

Acting

Man at Transvestite's Suicide (uncredited)

George Weiss

Production

Sheila, Glen's Sister (uncredited)

Evelyn Wood

Acting